estrazione psilocina senza ultrasuoni

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mirkodls
Messaggi: 102
Iscritto il: dom apr 18, 2021 11:08 am

estrazione psilocina senza ultrasuoni

Messaggio da mirkodls » lun mag 16, 2022 3:44 am

non riesco a trovare pressoche nulla sull'estrazione dei principi attivi dai funghi l'unica cosa e' con gli ultrasuoni cosa che gia faccio ma volevo sapere se qualcuno conosce o ha dei libri o qualsiasi cosa sull'estrazione dai vegetali e funghi. nel libro di hofmann parla dell'estrazione dai funghi messicani e della sintetizzazione del principio attivo quindi la cosa e' fattibile ma come??

ho fatto dei test con alcool 95 quello dei liquori poi messo a bagnomaria ad evaporare ma la soluzione e' inpura e si perde un mare di roba nelle filtrazioni ecc quindi non bene o almeno non va come ho provato io se potete darmi delle delucidazioni una sorta di 'ricetta' procedimento usato nei laboratori che studiano queste cose. con gli ultrasuoni anche la cosa non e' purissima e non forma cristalli come dovrebbe essere



mirkodls
Messaggi: 102
Iscritto il: dom apr 18, 2021 11:08 am

Re: estrazione psilocina senza ultrasuoni

Messaggio da mirkodls » lun mag 16, 2022 4:13 am

Experimental Procedure
A representative sample of 2 to 10g of dried mushrooms is ground to a fine powder by
mortar and pestle. The powder is mixed with 100 mL of dilute acetic acid in a 250-mL
beaker. The pH is readjusted to pH 4 with glacial acetic acid. After standing 1 h, the beaker
is placed in a boiling water bath for 8 to 10 min or until the internal temperature of the acid
mixture reaches 70~ The beaker is removed and cooled to room temperature under running water. The acid mixture is separated from the mushroom powder by suction filtration
using glass wool. The filtrate is brought to pH 8 with concentrated ammonium hydroxide
and quickly extracted with two 50-mL portions of diethyl ether. Gentle mixing instead of
shaking should be used to prevent an emulsion. The ether is dried over sodium sulfate, filtered, and evaporated under nitrogen with no applied heat.
Crude psilocin will appear as a greenish residue. Recrystalization from chloroform/nheptane (1 : 3) yields white crystals. The resulting powder can then be submitted to infrared
and mass spectral analyses.
Infrared spectra were obtained from potassium bromide disks on a Beckman Microlab
600 spectrophotometer. A Finnigan Model 3200 GC/MS with Model 6000 data system was
used for producing the mass spectra.
Results and Discussion
This method permits rapid isolation of psilocin from hallucinogenic mushrooms by coextraction of both psilocin and psilocybin. Dilute acetic acid is an excellent solvent for this
purpose, because both compounds are very soluble in acetic acid [11] and very little of other
interfering substances are extracted. It is most likely some other compounds are coextracted
but are removed from psilocin in the ether extraction from the aqueous base. Psilocybin is
completely dephosphorylated to psilocin by heating the acid extract. After addition of the
base, extraction into ether should be performed promptly, because of decomposition of psilocin at a greater than 7 [12]. The extraction and dephosporylation steps produce reasonably
pure psilocin from a small amount of mushroom material. Two grams of mushrooms will
often be sufficient to obtain an infrared spectrum of psilocin (Fig. 1). Smaller mushrooms
exhibits provide ample psilocin for mass spectral analysis (Fig. 2).
This method has been used in our laboratory for six months and has given excellent results
in seperating psilocin from methanol-soluble compounds. Other identification techniques
such as gas chromatography and microcrystalline tests are possible on psilocin extracted by
this method.

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Sagittarius-A
Messaggi: 13
Iscritto il: ven feb 11, 2022 8:38 pm

Re: estrazione psilocina senza ultrasuoni

Messaggio da Sagittarius-A » gio mag 19, 2022 12:26 am

Stavo seguendo questo thread tempo fa:
https://future4200.com/t/a-b-extraction ... /84573/553

Sembra molto interessante

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